%0 Generic %A Bhagwat, Amar %A Lakdawala, Seema %D 2019 %T H1N1 pdm PA::GFP intracellular movement in Nocodazole (16 µm) treated A549 cells during infection %U https://nih.figshare.com/articles/dataset/H1N1_pdm_PA_GFP_intracellular_movement_in_Nocodazole_16_m_treated_A549_cells_during_infection/10250534 %R 10.35092/yhjc.10250534.v1 %2 https://nih.figshare.com/ndownloader/files/18508712 %2 https://nih.figshare.com/ndownloader/files/18508721 %2 https://nih.figshare.com/ndownloader/files/18508724 %2 https://nih.figshare.com/ndownloader/files/18508901 %2 https://nih.figshare.com/ndownloader/files/18508904 %2 https://nih.figshare.com/ndownloader/files/18508907 %2 https://nih.figshare.com/ndownloader/files/18508910 %2 https://nih.figshare.com/ndownloader/files/18508913 %2 https://nih.figshare.com/ndownloader/files/18508919 %2 https://nih.figshare.com/ndownloader/files/18508922 %K influenza virus %K mircotubules %K Rab11A %K endosomes %K assembly %K RNA %K live-cell imaging %K fluorescence mircoscopy %K viral RNA %K motor transport %K vesicle tracking %X
TIFF series of diSPIM datasets, fused and deconvolved, from A549 cells infected with H1N1 pdm PA::GFP and treated with nocodazole (16 µm). Each TIFF file is a full cell volume of 50 planes, with isotropic resolution of 330nm, each .zip file is an independent replicate with at least 1 or 2 cells analyzed for 3D tracking of cytoplasmic foci with 500 time stamps. These data correspond to Supplementary Movie 7 and analysis of these movies provided data presented in Figure 4 and Supplementary Figure 8.

Supplementary Movie 7: A549 cells infected with H1N1 pandemic influenza expressing GFP-tagged PA protein and treated with nocodazole (16 µm).

Figure 4: Effect of cytoskeleton depolymerization on motion dynamics of influenza vRNP in A549 cells.

Supplementary Figure 8: Impact of cytoskeletal depolymerizing drugs on the transport of PA::GFP.
%I The NIH Figshare Archive