GFP-Rab11A intracellular movement in Nocodazole (16 µm) treated A549 cells

2019-11-07T17:54:54Z (GMT) by Amar Bhagwat Seema Lakdawala
TIFF series of diSPIM datasets, fused and deconvolved, from A549 GFP-Rab11A cells treated with Nocodazole (16 µm). Each TIFF file is a full cell volume of 50 planes, with isotropic resolution of 330nm, each .zip file is an independent replicate with at least 1 or 2 cells analyzed for 3D tracking of cytoplasmic foci with 500 time stamps. These data correspond to Supplementary Movie 4 and analysis of these movies provided data presented in Figure 3 and Supplementary Figure 4.

Supplementary Movie 4: A549 GFP-Rab11A cells under nocodazole treatment.

Figure 3: Motion Dynamics of Rab11A under the effect of cytoskeletal depolymerizing drugs.

Supplementary Figure 4: Impact of skeletal depolymerizing drugs on the transport of Rab11A.