IFNg FCS files to support Figs 3 & 4 in "The ancestral environment shapes antiviral CD8+ T cell responses across generations"
Figure 3: F0 exposure to TCDD has developmental, but not transgenerational effects on the CD8+ T response to IAV in the lung of male TCDD lineage offspring
7-8 male mice from each treatment group were infected with IAV and the CD8+ T cell response in the lung was measured 8 days later.
(I) The flow plot depicts the mean percent of IFNg+CD8+ T cells in the lung of F1 offspring. (J) The mean number of IFNg+CD8+ T cells in the lung of F1 offspring. (K) The flow plots show the mean percent of IFNg+CD8+ T cells in the lung of F3 offspring. (L) The mean number of IFNg+CD8+ T cells in the lung of F3 offspring.
Figure 4: In the MLN, F0 AHR activation alters the CD8+ T cell response of female and male F1 but not F3 generation offspring. F1 and F3 generation offspring were generated as shown in Fig 1A. 7-9 adult F1 and F3 generation offspring from each treatment group were infected with IAV and MLN cells were harvested on day 8 (males) or 9 (females) post infection. The graphs depict the mean number of cells from vehicle and TCDD offspring found in the MLN on day 8 or 9 post IAV infection.
(C) The number of IFNg+CD8+ T cells in female F1 (left) and F3 (right) offspring.
(G) The number of IFNg+CD8+ T cells in male F1 (left) and F3 (right) generation offspring.Funding
Ah Receptor Dysregulation Alters Hematopoietic Stem Cells
National Institute of Environmental Health Sciences
Find out more...Transgenerational exposures as modifiers of host defense against infection
National Institute of Environmental Health Sciences
Find out more...Training in Environmental Toxicology
National Institute of Environmental Health Sciences
Find out more...The T32 Predoctoral Training Program in Immunology (PTPI)
National Institute of Allergy and Infectious Diseases
Find out more...MULTIDISCIPLINARY TRAINING IN PULMONARY RESEARCH
National Heart Lung and Blood Institute
Find out more...Environmental Agents as Modulators of Disease Processes
National Institute of Environmental Health Sciences
Find out more...History
Select an IC:
- ES - National Institute of Environmental Health Sciences (NIEHS)
Is this associated with a publication?
- Yes