14B CeA Opto GFP Data NEW.xlsx (335.71 kB)

CeA ChR2 Virus Expression Mapping and quantification

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posted on 17.04.2020 by Shelley Warlow, Erin Naffziger

This data quantifies the spread of ChR2 Virus injections into CeA, spread around the implanted fiber optic tip.

Dataset supporting: Warlow, S.M., Naffziger, E.E. & Berridge, K.C. The central amygdala recruits mesocorticolimbic circuitry for pursuit of reward or pain. Nat Commun 11, 2716 (2020). https:/doi.org/10.1038/241467-020-16407-1

Abstract: How do brain mechanisms create maladaptive attractions? Here intense maladaptive attractions were created in laboratory rats by pairing optogenetic channelrhodopsin (ChR2) stimulation of central nucleus of amygdala (CeA) in rats with encountering either sucrose, cocaine, or a painful shock-delivering object. Pairings made the respective rats pursue either sucrose exclusively, or cocaine exclusively, or repeatedly self-inflict shocks. CeA-induced maladaptive attractions, even to the painful shock-rod, recruited mesocorticolimbic incentive-related circuitry. Shock-associated cues also gained positive incentive value and were pursued. Yet the motivational effects of paired CeA stimulation could be reversed to negative valence in a Pavlovian fear learning situation, where CeA ChR2 pairing increased defensive reactions. Finally, CeA ChR2 valence could be switched to neutral by pairing with innocuous stimuli. These results reveal valence plasticity and multiple modes for motivation via mesocorticolimbic circuitry under the control of CeA activation.

Find other related resources in the collection here: https://doi.org/10.35092/yhjc.c.4939542

Funding

Cue-triggered Reward Seeking

National Institute on Drug Abuse

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Affective neuroscience of taste reactivity

National Institute of Mental Health

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Sensory Mechanisms and Disorders

National Institute on Deafness and Other Communication Disorders

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NIDA Training Program in Neuroscience

National Institute on Drug Abuse

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History

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  • MH - National Institute of Mental Health (NIMH)
  • DA - National Institute on Drug Abuse (NIDA)

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