Complete Dataset for "CexE is a Coat Protein and Virulence Factor of Diarrheagenic Pathogens" Rivas et al. Frontiers in Microbiology, 2020. doi: 10.3389/fmicb.2020.01374.

2020-06-10T20:13:25Z (GMT) by George Munson Zachary Rivas
This file contains the data used to generate graphs and survival curves in publication "CexE is a Coat Protein and Virulence Factor of Diarrheagenic Pathogens" Rivas et al. Frontiers in Microbiology, 2020. doi: 10.3389/fmicb.2020.01374.

Sheets of this dataset are labeled so that they correspond to the published figures. Please refer to the open access citation for complete Materials and Methods.

Ethics statement. All animal experiments were approved by and performed in accordance with the University of Miami Institutional Animal Care and Use Committee guidelines.

Fig2 CexE Localization
Quantification of western blots to determine the sub-cellular location of a CexE in ETEC strain H10407 and C. rodentium strain GPM1830 cultured in CFA, IMDM, or IMDM under hypoxia.

Fig3B CexE-TEM-1
Quantification of western blots to determine the sub-cellular location of a CexE-TEM-1 fusion ETEC strain GPM1163 (cexE::kan) transformed with pGPM1034bla (cexE-tem1) by Proteinase K digestion of intact or permeabilized cells.

Fig4 Bacterial Attachment
Adherence of wild-type and cexE mutants of enterotoxigenic E. coli (ETEC) and Citrobacter rodentium to HCT-8 cells in IMDM 10% fetal bovine serum. Data contains CFU counts of adherent strains GPM1168 (ETEC, wild-type) and GPM1163 (ETEC, cexE::kan), GPM1236 (ETEC, rns::kan), GPM1831a (C. rodentium, wild-type) and GPM1827a (C. rodentium cexE::kan).

Fig5 CexC mutants
Quantification of western blots to determine the sub-cellular location of CexE in cexC::kan mutants of ETEC and Citrobacter rodentium by Proteinase K digestion of intact or permeabilized cells. Also includes the results of complementation with CexC expression plasmids and vector controls.

Fig6 Infection (Adult)
Fecal shedding, intestinal loads, and histological scores of adult C57BL/6 mice orogastrically inoculated with 108 CFU Citrobacter rodentium strains GPM1831a (wild-type) or GPM1827b (cexE::kan). Fecal pellets were collected on the days indicated post inoculation. Intestinal tissue and contents were harvested 10 days post inoculation. Also included are cage groupings, the sex, and daily weight of each subject.

Fig7A Infection (infants)
Colon titers on days 2, 4, and 10 post orogastrical inoculation of 15-day old C57BL/6 mice with 107 CFU Citrobacter rodentium strains GPM1831a (wild-type) or GPM1827a (cexE::kan).

Fig7B Survival Curves
Data for survival curves of 15-day old C57BL/6 mice orogastrically inoculated with 107 CFU Citrobacter rodentium strains GPM1831a (wild-type) or GPM1827a (cexE::kan). "1" indicates death, "0" indicates censored.

SFig2 CexE variants
Multiple sequence alignment of CexE variants.

SFig3 CexE Localization
Quantification of western blots to determine the sub-cellular location of CexE in Citrobacter rodentium by Proteinase K digestion of intact or permeabilized cells.

SFig4A ETEC growth
Data for growth curves of ETEC strains H10407 and GPM1163 (cexE::kan) transformed with vector pNEB193 or pGPM1039-27D (cexE+). Strains were cultured aerobically in IMDM at 37°C.

SFig4B Citrobacter growth
Data for growth curves of C. rodentium strains GPM1831a and cexE mutants GPM1827a and GPM1827b cultured aerobically in LB at 37°C.

SFig4C Citrobacter growth
Data for growth curves of GPM1831a and cexCCr mutants GPM2002a and GPM2002b. C. rodentium strains cultured aerobically in LB at 37°C.

SFig5 Infection BL6 (cexE)
Fecal shedding, intestinal loads, and histological scores of adult C57BL/6 mice orogastrically inoculated with 108 CFU GPM1831a (wild-type Citrobacter rodentium) or GPM1827b (cexE::kan). Fecal pellets were collected on the days indicated post inoculation. Intestinal tissue and contents were harvested 10 days post inoculation.

SFig6 Infection BL6 (cexC)
Fecal shedding, intestinal loads, and histological scores of adult C57BL/6 mice orogastrically inoculated with 108 CFU GPM1831a (wild-type Citrobacter rodentium) or GPM2002b (cexC::kan). Fecal pellets were collected on the days indicated post inoculation. Intestinal tissue and contents were harvested 10 days post inoculation. The sex of each subject is also reported.

SFig7 Infection 129 (cexE)
Fecal shedding of 129X1/SvJ mice orogastrically inoculated with 1010 CFU GPM1831a (wild-type Citrobacter rodentium) or GPM1827a (cexE::kan). Fecal pellets were collected on the days indicated post inoculation.