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IFNg FCS files to support Figs 3 & 4 in "The ancestral environment shapes antiviral CD8+ T cell responses across generations"

dataset
posted on 16.09.2019 by Christina Post, Lisbeth A. Boule, Catherine Burke, Colleen O'Dell, Bethany Winans, B. Paige Lawrence

Figure 3: F0 exposure to TCDD has developmental, but not transgenerational effects on the CD8+ T response to IAV in the lung of male TCDD lineage offspring


7-8 male mice from each treatment group were infected with IAV and the CD8+ T cell response in the lung was measured 8 days later.


(I) The flow plot depicts the mean percent of IFNg+CD8+ T cells in the lung of F1 offspring. (J) The mean number of IFNg+CD8+ T cells in the lung of F1 offspring. (K) The flow plots show the mean percent of IFNg+CD8+ T cells in the lung of F3 offspring. (L) The mean number of IFNg+CD8+ T cells in the lung of F3 offspring.


Figure 4: In the MLN, F0 AHR activation alters the CD8+ T cell response of female and male F1 but not F3 generation offspring. F1 and F3 generation offspring were generated as shown in Fig 1A. 7-9 adult F1 and F3 generation offspring from each treatment group were infected with IAV and MLN cells were harvested on day 8 (males) or 9 (females) post infection. The graphs depict the mean number of cells from vehicle and TCDD offspring found in the MLN on day 8 or 9 post IAV infection.


(C) The number of IFNg+CD8+ T cells in female F1 (left) and F3 (right) offspring.

(G) The number of IFNg+CD8+ T cells in male F1 (left) and F3 (right) generation offspring.

Funding

Ah Receptor Dysregulation Alters Hematopoietic Stem Cells

National Institute of Environmental Health Sciences

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Transgenerational exposures as modifiers of host defense against infection

National Institute of Environmental Health Sciences

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Training in Environmental Toxicology

National Institute of Environmental Health Sciences

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Predoctoral Training Program in Immunology

National Institute of Allergy and Infectious Diseases

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MULTIDISCIPLINARY TRAINING IN PULMONARY RESEARCH

National Heart Lung and Blood Institute

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Environmental Agents as Modulators of Disease Processes

National Institute of Environmental Health Sciences

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  • ES - National Institute of Environmental Health Sciences (NIEHS)

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