IFNg FCS files to support Figs 3 & 4 in "The ancestral environment shapes antiviral CD8+ T cell responses across generations"

Figure 3: F0 exposure to TCDD has developmental, but not transgenerational effects on the CD8+ T response to IAV in the lung of male TCDD lineage offspring

7-8 male mice from each treatment group were infected with IAV and the CD8+ T cell response in the lung was measured 8 days later.

(I) The flow plot depicts the mean percent of IFNg+CD8+ T cells in the lung of F1 offspring. (J) The mean number of IFNg+CD8+ T cells in the lung of F1 offspring. (K) The flow plots show the mean percent of IFNg+CD8+ T cells in the lung of F3 offspring. (L) The mean number of IFNg+CD8+ T cells in the lung of F3 offspring.

Figure 4: In the MLN, F0 AHR activation alters the CD8+ T cell response of female and male F1 but not F3 generation offspring. F1 and F3 generation offspring were generated as shown in Fig 1A. 7-9 adult F1 and F3 generation offspring from each treatment group were infected with IAV and MLN cells were harvested on day 8 (males) or 9 (females) post infection. The graphs depict the mean number of cells from vehicle and TCDD offspring found in the MLN on day 8 or 9 post IAV infection.

(C) The number of IFNg+CD8+ T cells in female F1 (left) and F3 (right) offspring.

(G) The number of IFNg+CD8+ T cells in male F1 (left) and F3 (right) generation offspring.