Plasmid maps and sequences.docx (274.51 kB)

Maps and full sequences of bacterial expression plasmids pTags2, pTags2-Hyg1, and pTags2-Zeo1

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posted on 11.06.2020 by George Munson
High copy number vectors for expression of one to six epitope tagged polypeptides from the Lac promoter in Escherichia coli and related species of bacteria. Expression is typically leaky but can be further increased with the addition of IPTG. Each epitope is preceded by its own Shine-Dalgarno sequence and start ATG codon. Three also have signal peptides; OmpA, PelB, and MalE. Recommended cloning method is by fragment assembly.

Supports publication: "CexE is a Coat Protein and Virulence Factor of Diarrheagenic Pathogens" Rivas et al. Frontiers in Microbiology, 2020. doi: 10.3389/fmicb.2020.01374.

Funding

Characterization Of A Novel ETEC Virulence Factor And Secretion System

National Institute of Allergy and Infectious Diseases

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