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Supplemental Figure 1 for Leucine augments specific skeletal muscle mitochondrial respiratory pathways during recovery following 7 days of physical inactivity in older adults

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posted on 18.03.2021, 22:24 by Emily Arentson-Lantz, Jasmine Mikovic, Nisha Bhattarai, Christopher S. Fry, Severine Lamon, Craig Porter, Doug Paddon-Jones
Descriptive schematics of high resolution respirometry protocols for measure skeletal muscle mitochondrial respiration in skeletal muscle of older adults.

Supplemental Figure supporting publication:

"Leucine supplementation augments skeletal muscle mitochondrial respiration during recovery following 7 days of physical inactivity in older adults"
Emily J. Arentson-Lantz, Jasmine Mikovic, Nisha Bhattarai, Christopher S. Fry, Severine Lamone, and Doug Paddon-Jones

In older adults, leucine supplementation attenuates the loss of skeletal muscle mass and function during bed rest. We sought to determine if leucine could also preserve and/or restore mitochondrial function and muscle oxidative capacity during periods of disuse and rehabilitation. Healthy older adults (69.1 ±1.1 years) consumed a structured diet with supplemental leucine (LEU: 0.06 g/ kg body weight/ meal; n=8) or alanine (CON: 0.06 g/ kg body weight/meal; n=8) during 7 days of bed rest and 5 days of inpatient rehabilitation. A 75 g oral glucose tolerance test was performed at baseline (PreBR), after bed rest (PostBR) and rehabilitation (PostRehab) and used to calculate an indicator of insulin sensitivity, metabolic clearance rate. (MCR). Tissue samples from the m. vastus lateralis were collected PreBR, PostBR, and PostRehab to assess mitochondrial respiratory capacity and protein markers of the oxidative phosphorylation and a marker of the antioxidant defense systems. During bed rest, leucine tended to preserve insulin sensitivity (Change in MCR, CON vs. LEU: -3.5 ± 0.82 vs LEU: -0.98 ± 0.88, p=0.054), but had no effect on mitochondrial respiratory capacity (Change in State 3+succinate CON vs. LEU -3.3 ± 6.1 vs. 7.3 ± 4.1 pmol O2/sec/mg tissue, p=0.19) Following rehabilitation, leucine increased ATP-linked respiration (CON vs. LEU: -8.9 ± 6.2 vs. 18.8 ± 3.7 pmol O2/sec/mg tissue, p=0.014). While the expression of mitochondrial respiratory and antioxidant proteins was not impacted, leucine supplementation preserved specific pathways of mitochondrial respiration, insulin sensitivity and a marker of oxidative stress during bed rest and rehabilitation.

Funding

Preserving muscle mass and function in bedridden older adults

National Institute of Nursing Research

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UTMB Claude D. Pepper Older Americans Independence Center

National Institute on Aging

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UTMB Clinical and Translational Science Award

National Center for Advancing Translational Sciences

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UTMB Clinical and Translational Science Award

National Center for Advancing Translational Sciences

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