Supplemental Figure 4: MEDIPS saturation plots of chicken embryonic gonads after in ovo exposure to ethynylestradiol
Supplemental Figure 4 supporting: Keiko Shioda et al. “Transcriptomic and epigenetic preservation of genetic sex identity in estrogen-feminized male chicken embryonic gonads”
Supplemental Figure 4: MEDIPS saturation plots.
Chicken embryos (29 total) were exposed to 20 microgram/egg ethynylestradiol or vehicle oil emulsion from incubation day 6, and their gonads were collected on day 19. Genomic DNA of these gonads were subjected to the MBD-seqs method of genomic DNA methylation profiling using deep sequencing. This figure shows saturation plot anlaysis performed for gonads showing various degrees of feminization [feminization scores (FS) = 0,1,2,3, and FS=3 ovaries exposed to ethynylestratiol (EE2)] using the bioconductor package MEDIPS.
Funding
Roles of Nuclear Receptors in Generation of Heritable Epimutations in Germ Cells
National Institute of Environmental Health Sciences
Find out more...Transgenerational obesity caused by ancestral exposure to obesogens in utero: changes in germline genomic architecture, roles of gonadal somatic cells, and metabolomic analysis of sexual dimorphism
National Institute of Environmental Health Sciences
Find out more...Developmental Gene-Environment Interactions and Premature Ovarian Failure
National Institute of Environmental Health Sciences
Find out more...History
Select an IC:
- ES - National Institute of Environmental Health Sciences (NIEHS)
Is this associated with a publication?
- Yes
I confirm there is no human identifiable information in this dataset.
- Yes
