Supplemental Figure 4: MEDIPS saturation plots of chicken embryonic gonads after in ovo exposure to ethynylestradiol

Supplemental Figure 4 supporting: Keiko Shioda et al. “Transcriptomic and epigenetic preservation of genetic sex identity in estrogen-feminized male chicken embryonic gonads”
Supplemental Figure 4: MEDIPS saturation plots.

Chicken embryos (29 total) were exposed to 20 microgram/egg ethynylestradiol or vehicle oil emulsion from incubation day 6, and their gonads were collected on day 19. Genomic DNA of these gonads were subjected to the MBD-seqs method of genomic DNA methylation profiling using deep sequencing. This figure shows saturation plot anlaysis performed for gonads showing various degrees of feminization [feminization scores (FS) = 0,1,2,3, and FS=3 ovaries exposed to ethynylestratiol (EE2)] using the bioconductor package MEDIPS.