Suppl_Fig4_MEDIPS_saturation_plots.pdf (648.79 kB)

Supplemental Figure 4: MEDIPS saturation plots of chicken embryonic gonads after in ovo exposure to ethynylestradiol

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posted on 20.10.2020, 21:28 by Toshihiro Shioda, Keiko Shioda, Junko Odajima, Bianca Cordazzo, Misato Kobayashi, Mutsumi Kobayashi

Supplemental Figure 4 supporting: Keiko Shioda et al. “Transcriptomic and epigenetic preservation of genetic sex identity in estrogen-feminized male chicken embryonic gonads”
Supplemental Figure 4: MEDIPS saturation plots.

Chicken embryos (29 total) were exposed to 20 microgram/egg ethynylestradiol or vehicle oil emulsion from incubation day 6, and their gonads were collected on day 19. Genomic DNA of these gonads were subjected to the MBD-seqs method of genomic DNA methylation profiling using deep sequencing. This figure shows saturation plot anlaysis performed for gonads showing various degrees of feminization [feminization scores (FS) = 0,1,2,3, and FS=3 ovaries exposed to ethynylestratiol (EE2)] using the bioconductor package MEDIPS.

Funding

Roles of Nuclear Receptors in Generation of Heritable Epimutations in Germ Cells

National Institute of Environmental Health Sciences

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Transgenerational obesity caused by ancestral exposure to obesogens in utero: changes in germline genomic architecture, roles of gonadal somatic cells, and metabolomic analysis of sexual dimorphism

National Institute of Environmental Health Sciences

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Developmental Gene-Environment Interactions and Premature Ovarian Failure

National Institute of Environmental Health Sciences

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