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H1N1 pdm PA::GFP intracellular movement in Latrunculin A (1 µm) treated A549 cells during infection

dataset
posted on 07.11.2019 by Amar Bhagwat, Seema Lakdawala
TIFF series of diSPIM datasets, fused and deconvolved, from A549 cells infected with H1N1 pdm PA::GFP and treated with latrunculin A (1 µm). Each TIFF file is a full cell volume of 50 planes, with isotropic resolution of 330nm, each .zip file is an independent replicate with at least 1 or 2 cells analyzed for 3D tracking of cytoplasmic foci with 500 time stamps. These data correspond to Supplementary Movie 8 and analysis of these movies provided data presented in Figure 4 and Supplementary Figure 8.

Supplementary Movie 8: A549 cells infected with H1N1 pandemic influenza expressing GFP-tagged PA protein and treated with latrunculin A (1 µm).

Figure 4: Effect of cytoskeleton depolymerization on motion dynamics of influenza vRNP in A549 cells.

Supplementary Figure 8: Impact of cytoskeletal depolymerizing drugs on the transport of PA::GFP.

Funding

Visualization of Influenza Viral RNA Assembly

National Institute of Allergy and Infectious Diseases

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Visualizing Influenza Viral RNA Packaging

National Institute of Allergy and Infectious Diseases

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The Role of Pathogen-Environment Interactions in the Pandemic Potential of Influenza

National Institute of Allergy and Infectious Diseases

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Intramural research program of the National Institute of Biomedical Imaging and Bioengineering (NIBIB)

American Lung Association Biomedical Research Award (RG-575688)

New Initiative Award (KA2018-98552) from the Charles E. Kaufman Foundation, a supporting organization of The Pittsburgh Foundation

History

Select an IC:

  • AI - National Institute of Allergy and Infectious Diseases (NIAID)

Is this associated with a publication?

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I confirm there is no human identifiable information in this dataset.

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