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Sequencing result of a mixture of synthesized DNA barcodes

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posted on 19.12.2019 by Du Jiang

Raw data for the Nature Protocol manuscript entitled "clonal tracking using embedded viral barcoding and high-throughput sequencing".


Embedded viral barcoding in combination with high throughput sequencing is a powerful technology to track single cell clones. It can provide clonal level insights into cellular proliferation, development, differentiation, migration, and treatment efficacy.


Here, we present a detailed protocol of a viral barcoding procedure including the creation of barcode libraries, the viral delivery of barcodes, the recovery of barcodes, and the computational analysis of barcode sequencing data. The entire procedure can be

completed within a few weeks.


This barcoding method requires cells to be susceptible to viral transduction. It provides high sensitivity and throughput, and allows precise quantification of cellular progeny. It is cost efficient and does not require any advanced skills. It can also be easily adapted to many types of applications, including both in vitro and in vivo experiments.



Funding

Lineage bias and clonal expansion of hematopoietic stem cell differentiation

National Heart Lung and Blood Institute

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Investigating the heterogeneous aging of individual hematopoietic stem cell clones

National Heart Lung and Blood Institute

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Tracing the developmental origin of hematopoietic stem cell heterogeneity

National Heart Lung and Blood Institute

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History

Select an IC:

  • HL - National Heart, Lung, and Blood Institute (NHLBI)

Is this associated with a publication?

Yes

I confirm there is no human identifiable information in this dataset.

Yes

Licence

Exports