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Two-color imaging of GFP-Rab11A and WSN H1N1 PA::mRuby in IAV infected A549 cells

dataset
posted on 07.11.2019 by Amar Bhagwat, Seema Lakdawala
TIFF series of diSPIM datasets, fused and deconvolved, from A549 GFP-Rab11A cells infected with WSN H1N1 PA::mRuby influenza virus. Each TIFF file is a full cell volume of 50 planes, with isotropic resolution of 330nm, each .zip file is an independent replicate with at least 1 or 2 cells analyzed for 3D tracking of cytoplasmic foci with over 100 time stamps. These data correspond to Supplementary Movie 11 and analysis of these movies provided data presented in Figure 6 and Supplementary Figure 11.

Supplementary Movie 11: A549 GFP-Rab11A infected with PA::mRuby virus.

Figure 6: Two-color imaging of PA::mRuby infected GFP-Rab11A A549 cells.

Supplementary Figure 11: Additional motion parameters for two-color imaging datasets.

Funding

Visualization of Influenza Viral RNA Assembly

National Institute of Allergy and Infectious Diseases

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Visualizing Influenza Viral RNA Packaging

National Institute of Allergy and Infectious Diseases

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The Role of Pathogen-Environment Interactions in the Pandemic Potential of Influenza

National Institute of Allergy and Infectious Diseases

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Intramural research program of the National Institute of Biomedical Imaging and Bioengineering (NIBIB)

American Lung Association Biomedical Research Award (RG-575688)

New Initiative Award (KA2018-98552) from the Charles E. Kaufman Foundation, a supporting organization of The Pittsburgh Foundation

History

Select an IC:

  • AI - National Institute of Allergy and Infectious Diseases (NIAID)

Is this associated with a publication?

Yes

I confirm there is no human identifiable information in this dataset.

Yes

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